Gilson 402 - Manual (Page 53)

5-3
Operation - Advanced Use
5
Correct Use of the 402
• If a drop of liquid is present at the end of the
tubing after dispensing, you must include this
drop in the result vial.
• The larger capacity syringe must always be
mounted on the left-hand side.
• Only the left-hand syringe aspirates the liquid
from the reservoir (diluent and rinsing liquid).
• If the capacity of the right-hand syringe is
superior to the total volume aspirated (volumes of
samples, reagents, air gaps), the right-hand
syringe aspirates and delivers liquids from the
probe.
• If the capacity of the right-hand syringe is inferior
to the total volume to be aspirated (including air
gaps), the left-hand syringe will be used to
aspirate all liquids.
• Syringe functionality is shown below,
steps (a) to (e).
Left-hand Syringe Aspirating (from
Reservoir)
1. The valve switches to connect the
left-hand syringe to the reservoir.
2. The piston of the right-hand syringe
remains in the top position.
3. The left-hand piston descends to
aspirate the specified volume of
liquid from the reservoir.
Left-hand Syringe Dispensing
(from Reservoir)
1. The valve switches to connect the
left-hand syringe to the Tee-junction
and probe.
2. The piston of the right-hand syringe
remains in the top position to
prevent liquid from entering the
syringe.
3. The left-hand piston ascends to
dispense the specified volume of
liquid from the reservoir.
(a) Left-hand Syringe
Aspirating from Reservoir
Left piston
descends
to aspirate Right piston
remains at
the top
From Reservoir
(b) Left-hand Syringe
Dispensing
Left piston
ascends
to dispense Right piston
remains at
the top
To Tee To Probe