Gilson 402 - Manual (Page 58)

5-8
Operation - Advanced Use
5
When you specify left and right syringe capacities
(left must be > right), you must enter compatible
volumes. An error message is displayed if you try
to enter an incompatible volume, in which case
you may need to change the volume of the other
syringe, before entering the volume of the one
that provoked the error message.
• Correction Factor
To compensate for the effects of temperature and
viscosity, a factor may be applied to all liquid
volumes. This factor should be calculated from
gravimetric assays (see page 5-33).
With the Dual-syringe model, you must first
select the syringe to which the correction applies.
Starting from the Configuration Screen, press Syr,
then use Next (or Prev) to select the right-hand or
left-hand syringe.
Press Corr, you then see the Syringe Correction
Screen. Key in a factor between 0 and 9.9 (%), press
ENTER to confirm. Press ESC, then use Prev (or
Next) to select the other syringe (if fitted), enter
the correction in the same way; press ESC (twice)
to return to the Configuration Screen.
Flow Rates
Press the Flow soft-key; the Air Flow Screen appears.
Air Flow is the rate in ml/min. that air is drawn
into the transfer tubing when aspirating air.
Key in the required value, then press ENTER. Or,
press Deflt to use the default value (4 mL/min).
Press the ESC key to return to the Configuration
Screen or press Next to go to the Resv Flow Screen.
‘Resv asp:’ is the rate in mL/min. that solvent is
aspirated from the reservoir by the left syringe.
Key in the required value, then press ENTER. Or,
press Deflt to use the default value. Press the ESC
key to return to the Configuration Screen or press
Next to go to the Prime Flow Screen.
Prime Flow is the rate in mL/min. that liquid is
transferred by the syringe(s) when priming.
Key in the required value, then press ENTER. Or,
press Deflt to use the default value. Press the ESC
key to return to the Configuration Screen. The flow-
rate limits depend on the volume of the syringe(s)
and type of tubing used, see pages 5-31 and 5-32.
Software
When you turn the syringe
correction factor ON, a plus sign
(+) appears after the file number,
as a reminder.
Syringe Correction Screen
Air Flow Screen
Resv Flow Screen
Prime Flow Screen