Gilson 402 - Manual (Page 70)

5-20
Operation - Advanced Use
5
For each dilution in the series:
Vt = Vs x Rd, Vd = Vt - Vs
For example, after selecting Dilution Ratio Mode, you
enter Sample Volume = 10 and Dilution Ratio = 3;
the software calculates the Total Volume as 30 and
the required Diluent Volume as 20.
1. When diluent comes from the reservoir (RESV);
aspirate the required volume of diluent (for all
dilutions, up to the capacity of the left-hand
syringe). If diluent comes from the probe, an air
gap is aspirated before the diluent, and the
required volume of diluent is aspirated (for all
dilutions, up to the capacity of the right-hand
syringe, after which the left-hand syringe is used).
2. Aspirate an air gap (specified in configuration).
3. Aspirate sample.
4. Dispense all into the recipient vessel.
5. Aspirate an air gap (specified in configuration).
6. Aspirate diluted sample.
7. Dispense all into the recipient vessel.
8. Aspirate an air gap (specified in configuration).
9. Aspirate diluted sample.
10. Dispense all into the recipient vessel.
11. And so on ...
Typical Task Entry
Key to press:
Key in the File # (e.g. 48),
Press ENTER
Press Edit
Press Dil
Press Serial
Press Chge to select source, press ENTER, then
Next.
Press Chge to select
mode, press ENTER,
Press Next, key in a
number of serial
dilutions, press ENTER.
Press Next, then key in the sample volume, press
ENTER.
Depending on the
mode, you see one of
three screens after
pressing Next.
Key in the value, press ENTER.
Software
Screens: