Gilson 402 - Manual (Page 75)

5-25
Operation - Advanced Use
5
Press ESC (twice).
Press ESC.
Pipette
You use this task to aspirate a series of fractions via
the probe, then dispense the total volume aspirated
into a recipient vessel. Each liquid aspirated is sepa-
rated by an air gap (if air gap is configured as > 0).
You may also aspirate an ‘Extra volume’ to ensure the
accuracy of the first fraction. This ‘Extra volume’,
which is aspirated with the first fraction, is dispensed
at the end of the process, into the waste.
1. Aspirate an air gap.
2. Aspirate the first fraction (with
‘Extra volume’, if specified).
3. Aspirate an air gap.
4. Aspirate the second fraction.
5. Aspirate an air gap.
6. Aspirate the third fraction.
7. Dispense all except ‘Extra volume’
into recipient vessel.
8. Dispense ‘Extra volume’ to waste.
Key to press
Key in the File # (e.g. 45), ENTER
Press Edit,
Press Pip, then select one of two modes, where:
(a) Fractions are equal,
(b) Fractions are different.
Press Chge to switch
modes. Press ENTER,
then Next.
Key in number of
fractions,
press ENTER, then
Next.
For SAME mode, key
in common volume,
for DIFFERENT
mode, key in volume
for fraction 1, press
Next, key in volume
for fraction 2, Next, etc.
Press ENTER, then Next.
Software
When you configure disposable
tips in air mode, ‘Extra volume’
is replaced by ‘Air push volume’
in this task.
1
air gap 2
fraction 1 3
air gap
4
fraction 2
5
air gap
6
fraction 3
7
result