Operation - standard use, Prime the 402 (man) – Gilson 402 User Manual

Page 46

Advertising
background image

4-10

Operation - Standard Use

4

Prime the 402 (Man)

Prime is used to thoroughly clean out all fluid

pathways with fresh solvent and to clear bubbles

from the tubings.

Press the Man soft-key, the Manipulation Screen

appears.

Press Prime, Initialization Screen 2 appears.

[Measure and Titrate are described in Chapter 5.]

You should prime the 402 before each run, to

ensure that all fluid pathways contain fresh solvent

and no air bubbles.

Put the probe-end of the transfer-tubing into a

suitable the waste container, then press OK.

Priming starts immediately and the message

‘Priming ...’ appears on the screen; during which

time solvent is expelled at the probe-end of the

transfer tubing.

Press STOP, or the End soft-key, when you are

satisfied that all fluid pathways have been primed

with fresh solvent and are free of air bubbles.

Pressing End

When you press End, the motion of the syringe(s)

ceases and ‘Homing ...’ appears on the screen until

the syringe(s) have returned to their uppermost

positions.

Pressing STOP

You can press STOP at any time, however, this key

is more for use if a problem arises (such as a leak).
When you press STOP, the motion of the syringe(s)

ceases and the Abort Screen appears. Press Proceed,

when you want to continue the prime from the

point at which it was stopped (for example, to refill

the solvent reservoir).
Press Abort, when you want to stop priming;

Initialization Screen 2 reappears. Ensure that the

probe-end of the transfer-tubing is in a suitable

waste container, then press OK.

Initialization Screen 2

Manipulation Screen

Essential Steps

Abort Screen

Advertising