Gilson 402 - Manual (Page 73)

5-23
Operation - Advanced Use
5
If for any reason you want to repeat the measure-
ment (e.g. you need to select the LEFT syringe
instead of the RIGHT), put tip of the transfer-
tubing back into the original container, then press
Redo to discharge the liquid.
When you are ready
to dilute the previ-
ously measured
sample, press End.
The command light is
steady and you see
alternately the next
pair of screens.
Press the command
button, diluent is drawn
from the reservoir; the
command light is
steady and you see
alternately the next
pair of screens.
Put the tip of the transfer-tubing back into a suitable
recipient vial then press the command button to
dispense the diluted sample. After which you are
returned to the following screen for the next cycle.
If you want to quit at this point you must press the
STOP key.
Dispense
This task is used to dispense liquid from the
reservoir or from the probe, in equal or un-
equal fractions, into one or more vessels. You
may adjust the final volumes in the recipient
vessels to a predetermined final volume, with
liquid via the reservoir or the probe. If the
source is the reservoir, you can dispense a
volume greater than the capacity of the left-
hand (or only) syringe, which will complete
this operation with several strokes of the piston.
When you use this task to aspirate
and dispense via the probe, you may
also aspirate an ‘Extra volume’. The
‘Extra volume’ is aspirated to ensure
that the accuracy of the first volume
of liquid is maintained (when it is
dispensed) and that the first fraction
is not contaminated by liquid coming
from the reservoir. The ‘Extra volume’,
which is aspirated with the first
Software
Adjust 1
Fraction 1 Fraction 2 Fraction 3
Adjust 2 Adjust 3
Final
Volume
1
air gap
2
liquid
3
fraction1
4
fraction 2
5
fraction 3
6
fraction 4
7
to waste
Extra Volume