Metrohm IC Net 2.0 - Manual (Page 129)

7.5 Calibration and quantification
IC Net 2.0 121
7.5.3 External standard calibration
External standard calibration (absolute calibration) is a key way of
calibration. Basically this procedure calculates the dependence
of injected component's quantity versus area (or height) of the
corresponding peak. This dependence is showed as a calibration
plot with injected quantity along the Y axis and peak area (or
height) along the X axis. The injected quantity Q
i
is calculated as
product of component's concentration C
i
to the reduced injected
sample volume V':
Q
i
= C
i
• V'
The calibration function W
i
(R)
for each component is calculated
using the RSD method.
W
i
(R) = RSD(Q
ij
, R
ij
) = RSD((C
i
• V'), R
ij
)
When quantification procedure is performed, a concentration C
i
(absolute concentration of a component in the sample) is deter-
mined as a ratio of the computed quantity W
i
(R
i
)
to the corrected
volume of sample injected V':
C
i
= W
i
(R
i
) / V'
7.5.4 Component table
IC NET / Method / Calibration / Components
If the component table is opened, the chromatogram window is
split into two parts. The upper one shows the chromatogram, the
component table appears in the bottom part of the screen. When
moving into the component table, a special cursor in the upper
part of the window jumps to the peak corresponding to the cur-
rent component.
The component table stores the following information on the
components to be analyzed: