Metrohm IC Net 2.0 - Manual (Page 150)

7 Methods
IC Net 2.0 142
effectivity, TP
Effectivity for the peak in number of theo-
retical plates. The number of theoretical
plates N
i
per column for a chosen peak is
calculated for a chromatographic peak by
one of two formulas:
N
i
= 2 PI (t
i
• H
i
/ A
i
)
2
,
where PI = 3.1415926..., t
i
= retention
time, H
i
= height, A
i
= area of the peak.
The more commonly used formula is:
N
i
= 5.54 (t
i
/ w
i
)
2
,
where w
i
is the width on the half-height of
the peak. The first formula offers better es-
timates for fused or unresolved peaks, be-
cause the half-width errors for those peaks
are much greater than height or area errors.
Total for this column includes average
value for the peaks listed.
effectivity, TP/m Effectivity for the peak in number of theo-
retical plates per meter. The number of
theoretical plates per meter N' for the given
component is calculated as:
N' = N
i
• 1000 / L,
where L is length of the column in mm and
N
i
is effectivity of the column for i-th com-
ponent.
Total for this column includes average
value for the peaks listed.
reduced TP height, HETP/dp
The height of theoretical plate divided by
particle size, called also reduced height, is
calculated by formula:
H
i
= 1000 • L / (N
i
dp).
where L is length of the column in mm, dp
is particle diameter in
µ m.
asymmetry Peak asymmetry A
s
is calculated at 1 /
10
of
the peak height as a ratio of width after the
top of the peak w
2
to the width before the
top w
1
.
A
s
= w
2
/ w
1
response factor
Coefficient k
1
of the calibration curve.
raw concentration Absolute concentrations (raw quantity) of
the components, calculated as
C
i
= W
i
(R
i
) / V'