HP Data Explorer 4 Series - Manual (Page 338)

Chapter 7 Data Explorer Examples
7-22 Applied Biosystems
7
Figure 7-22 Deisotoping Dialog Box
10. For this example spectrum, specify H for Adduct and
C6H5NO for Generic Formula.
11. Click OK. For more information on deisotoping, see
Section 3.4, Deisotoping a Spectrum.
Increasing
detection
thresholds
12. In the Basic Settings tab in Global Thresholds (see
Figure 7-20 on page 7-20), increase %Max Peak Area
to a setting between 0.1 and 1% until the peaks of
interest are detected and noise is screened out.
Figure 7-23 shows the trace after deisotoping. Note
that many fewer peaks are detected. By further
fine-tuning %Max Peak Area, you can screen out
additional noise peaks.
Figure 7-23 Digest After Noise Filtering, Deisotoping,
and Adjusting Detection Thresholds