C.7 using ms fit/ms tag toolbox, Using ms fit/ms tag toolbox – HP Data Explorer 4 Series User Manual

Page 414

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Appendix C Data Explorer Toolbox (Visual Basic Macros)

C-18

Applied Biosystems

C

C.7 Using MS Fit/MS Tag Toolbox

Use the MS Fit/MS Tag toolbox when analyzing protein digest,
peptide, or peptide fragment spectra to perform a protein
database search.

Preparing data

before accessing

Before using the MS Fit/MS Tag toolbox:

1.

In the Data Explorer software, smooth the data, perform

a baseline correction, then deisotope.

2.

If needed, use Advanced Peak Detection parameters to
adjust peak detection ranges and thresholds to screen out
noise in low-mass regions and to detect peaks in
high-mass regions.

3.

Set the Set the %Base Peak Intensity or %Max Peak Area
in peak detection until the unwanted noise peaks are
removed from the Spec Peak list.

Running

MS Fit/MS tag

To run the MS Fit/MS Tag macro:

1.

In the Toolbox Palette dialog box, click MS Fit/MS Tag.

Figure 3-15 MS Fit Dialog BoxMS Tag Dialog Box

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