2 general information on separating columns, 3 selecting the sample loop, General information on separating columns – Metrohm 861 Advanced Compact IC User Manual
Page 41: Selecting the sample loop
2.7 Precolumns and separating columns
861 Advanced Compact IC / Instructions for Use 8.861.1033
31
1 Connect precolumn
•
Remove end caps from the precolumn.
•
Fit compression fitting to the connection capillary 26 moun-
ted to the injection valve (see Section 2.3.5).
•
Screw column connection capillary 26 to precolumn.
•
Cut off a small piece, approx. 5 cm, from the 6.1831.010
PEEK capillary and fit compression fittings to both ends (see
Section 2.3.5).
•
Mount the prepared capillary to the outlet of the precolumn.
When you install the column always ensure that this is inserted cor-
rectly in accordance with the flow direction (if existent) shown.
2 Rinse the precolumn
•
Place a beaker beneath outlet capillary.
•
Switch on IC Pump in «IC Net» and rinse precolumn for
approx. 10 min with eluent.
•
Switch off IC Pump.
2.7.2 General
information
on separating columns
The precolumn that is suitable for your separating column can be
found in the
Metrohm IC Column Catalog, the product information
about separating columns that can be found under
on chromatography products, or let your agency advise
you directly.
New IC columns are normally supplied filled with liquid and sealed at
both ends. Before inclusion in the system you must make sure that
this solution is fully miscible with the eluent to be used (observe the in-
formation provided by the manufacturer).
The separating columns and precolumns currently available from
Metrohm can be found in the Metrohm IC Column Catalog, or on the
Internet under
duct section. Each column is supplied with a test chromatogram and a
data sheet. More detailed information about special IC applications can
be found in the relevant "Application Bulletins" or "Application No-
tes"; these are available on the Internet under
in the applications sector, or can be requested free of charge from your
local Metrohm agency.
When you install the column always ensure that this is inserted cor-
rectly in accordance with the flow direction shown.
2.7.3
Selecting the sample loop
Selection of the sample loop depends on the separating column used.
Normally the following sample loops are used: