ITC VerifyNow System Platelet Reactivity Test User Manual
Page 36
Patient Testing - 6
Page 32
14439.C 03/2013
VerifyNow System User Manual |
USA
6.2
Sample Collection
Samples can be collected from an indwelling catheter or using direct venipuncture.
Fill Volume
NOTE: Always ensure collection tubes are filled to the indicated fill volume.
NOTE: At altitudes greater than 2500 feet above sea level, blood collection
tubes may not fill to the specified volume, which results in an incorrect
ratio of blood to anticoagulant. Users at these elevations should use
tubes designed for high altitudes or refer to their facility’s blood collection
protocols for instructions to properly fill blood collection tubes.
Figure 6‑1 Sample Tube
6.2.1
Peripheral Samples
Perform the following procedure for sample collection directly into vacuum collection tubes:
NOTE: For an Aspirin or IIb/IIIa Test, whole blood may be collected from peripheral venous or arterial sites.
Materials
You will need the following materials:
•
Discard tube – may be a tube with no additive, sodium citrate, blood culture, or heparin tube (IIb/IIIa
test only). The tube cannot contain EDTA (purple top).
•
For the specific VerifyNow Test sample tube, refer to
Table 2-1 Sample Collection Tubes
•
1 Label with patient ID (optional)
Sample Collection Procedure
Collection of blood sample should be performed with care to avoid hemolysis or contamination by tissue
factors.
1.
Whole blood may be collected from venous sites using a 21 gauge or larger (e.g. 18-20 gauge)
needle in an appropriate blood collection tube. Blood samples should be obtained from an extremity
free of peripheral venous infusions.
2.
Collect a discard tube first (approximately 2 mL). The discard tube must not contain EDTA.
3.
If drawing for a CBC at the same time, fill the CBC tube
last to avoid contamination by EDTA.
4.
Fill the second tube (sample tube) to the black line (Figure 6-1). Do not under fill.
NOTE: Label the sample tube with the patient ID, date, and time it was drawn. Dispose of blood collection supplies
according to your institution’s policy.
5.
Discard the first tube in a biohazard waste container. Keep the second tube for testing. Label the
second tube with the patient ID, date, and time it was drawn. Dispose of blood collection supplies
according to your institution’s policy.