Dako Autostainer - Manual (Page 103)

Dako Autostainer | User Guide 103
Section 19 | Wash Buffers
Wash buffers are required for all immunohistochemical staining procedures. They function to eliminate
background by washing away the nonspecifically bound proteins from the specimen. Commonly used
buffers for immunohistochemical staining are Tris-buffered saline (TBS) or phosphate buffered saline
(PBS). These buffers are recommended by Dako for use on the Dako Autostainer. The following
pages describe detailed instructions for preparation of TBS and PBS buffer solutions. Buffers used
with the Dako Autostainer should contain a final concentration of 0.05% Tween 20.
Dako Autostainer Wash Buffer
The Dako Autostainer Wash Buffer is a Tris-buffered saline solution (TBS), pH 7.6, containing 0.05%
Tween 20. It is recommended that Dako prepackaged buffer solutions are used for consistent
performance.
Intended Use
Dako Tris-Buffered Saline Solution containing 0.05% Tween 20 is used as the standard wash
buffer for the Dako Autostainer.
Materials Required
1. Clean carboy, flask or beaker graduated to 1 Liter and 5 Liters
2. Deionized water
3. Magnetic stir plate with stir bar
4. Prepackaged Dako reagents
Code No. Contents
S3006 One Liter of Wash Buffer 10X. Each bottle makes 10 liters of 50mM Tris-
HCl, 150mM NaCl, 0.05% Tris-Buffered Saline containing Tween 20 and a
preservative, pH 7.6
or
S3001 Six packets of TBS containing Tris base, Tris-HCI and NaCl. Each packet
makes 1 liter of 50mM Tris-HCl, 150mM NaCl Tris-Buffered Saline, pH 7.6
or
S1968 Two packets of TBS containing Tris base, Tris-HCl and NaCl. Each packet
makes 5 liters of 50mM Tris-HCl, 150mM NaCl Tris-Buffered Saline, pH 7.6
or
S1966 Tween 20, 100 mL.