Dako Autostainer - Manual (Page 65)

Dako Autostainer | User Guide 65
Each cell on the screen represents a microscope slide. Cells contain the position number, case
number and primary antibody short name. If case number and primary antibody name have not
been assigned, the cells contain only the position number.
Slide Number
Position is located in the upper left corner and identifies the slide rack
position.
Primary Antibody
Dispense Volume is located in the upper middle section and displays the volume of
primary antibody to be dispensed on each highlighted section of
every slide.
N/P Notation is located in the upper right corner and displays a P or N if this
slide is programmed as a positive or negative control with the
Pos.Ctl or Neg.Ctl tool. (See Programming Slides, section 10)
Abbreviated Primary
Antibody Name
(Short Name) is located at the bottom and displays the primary antibody name.
Case Number is located in the middle and lists the Case # assigned to the slide.
Yellow Highlight
(Drop Zone) indicates the reagent dispense location. This bar appears on all
programmed slides.
Assigning Reagent Dispense Locations
The non-frosted area of each slide is equally divided into three horizontal sections or dispense
locations. Each slide can have one to three dispense locations. The maximum dispense
volume of a slide cannot exceed 800
µL (controlled by the software). The default dispense
location is programmed during initialization. Any modifications to the dispense volume or
dispense location of a slide in the SLIDE LAYOUT MAP:PROGRAM SLIDES screen will
change the text color of that slide from black to red.
1. Selecting a single dispense location:
Select a new reagent dispense location on each slide in the SLIDE LAYOUT MAP:
PROGRAM SLIDES screen by clicking on the desired dispense location using the mouse
pointer. The yellow highlight appears in the selected location on each programmed slide.
Slide Number
Position
Primary Antibody Dispense
Volume per highlighted drop zone
Abbreviated Primary
Antibody Name
(Short name)
Drop Zone
(yellow highlight)
Case Number (if text is too
long it wraps to the next line)
P or N signifies Positive or
Negative control