Dako Autostainer - Manual (Page 18)

18 Dako Autostainer | User Guide
14. Select the PRINT button to print the Reagent Layout Map.
15. Load reagents using the Reagent Layout Map.
16. Select the NEXT button. The SLIDE LAYOUT MAP:LOAD SLIDES screen appears.
17. Load slides using the Slide Layout Map. It is recommended that each slide be flooded
gently with buffer after it is placed on the instrument. This buffer should contain 0.05%
Tween 20. (See Loading Slides, Section 13) At no time should the slides dry out.
18. Select the NEXT button. The SET START TIME screen is displayed. (See Starting a Run,
Section 14)
19. Select the PRIME PUMP (WATER) button. The RUN PROGRAM NOW dialog box
appears. (Deionized water is not available with the “01” hardware version of the Dako
Autostainer). The user is prompted: “Is the pathway clear for the arm to move.”
20. Select the YES button and the pump is activated. Deionized water is flushed through the
water intake line.
21. Select the PRIME PUMP (BUFFER) button and the pump is activated, flushing buffer
through the buffer intake line. For the “01” hardware version of the Dako Autostainer the
user is prompted: “Is the pathway clear for the arm to move.”
22. Set Reagent Levels to Check volumes or Do not check volumes. (See Starting a Run,
Section 14)
23. Select the START RUN button and the run starts.