Dako Autostainer - Manual (Page 46)

46 Dako Autostainer | User Guide
Protocol Elements
The Protocol Elements are categories of reagents used to build the Protocol Outline in the
PROTOCOL TEMPLATE DESIGN screen. Reagents can be added, deleted and edited by choosing
Protocol Element categories from the Edit Lists menu item on the PROGRAMMING GRID.
Standard Protocol Elements include:
• Endogenous Enzyme Block
• Protein Block
• Primary Antibody
• Pretreatment
• Secondary Reagent
• Tertiary Reagent
• Labeled Polymer
• Substrate
• Auxiliary
Other Protocol Elements may be defined as steps in the template:
Rinse Buffer
A buffer rinse step consists of a buffer wash and air blow cycle and should be programmed
between each protocol template step. Each buffer rinse is displayed as a blue droplet in the
programming grid with a “droplet” icon in each programmed row. The buffer icon is printed out on
the Main Grid printout as a filled in droplet.
Rinse Water
A water rinse step consists of a water wash and air blow cycle and is an option that can be
selected in the Protocol Template. Each water rinse is displayed as a white droplet in the
programming grid with a “droplet” icon in each programmed row. The water icon is printed out on
the Main Grid printout as a white droplet.
NOTE: The rinse water option is not available with the “01” hardware configuration of the Dako
Autostainer.
Standard Rinse Replacement
The standard rinse cycle can be replaced by a blow only step (reagent is blown off the slide
but no buffer is applied) by clicking on the droplet icon in the programming grid using the right
mouse button. The droplet icon is replaced by a “blow” icon. The blow icon is printed out on the
Main Grid printout as a wind symbol.