Section 12 | loading reagents, Reagent layout map screen – Dako Autostainer User Manual

Page 79

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Dako Autostainer

|

User Guide

79




Section 12

|

Loading Reagents

After programming is completed the Autostainer calculates the time required for the staining run, the
amount of wash buffer/deionized water required and the most efficient placement for the reagent vials.

1. Select

the

NEXT button in the SLIDE LAYOUT MAP screen. A dialog box signaling that

the calculation is complete lists the run time, number of probe washes and the volume of
buffer and deionized water required. (The deionized water feature is not available with the
“01” hardware version of the Dako Autostainer.)






2. Select

the

OK button. A dialog box prompts “Save program on disk”.

3. Select

the

YES button and the “Save program on disk” window is displayed. Enter a name

for the program and select the OK button. The name cannot exceed 8 alpha-numeric
characters; symbols and spaces cannot be used. The REAGENT LAYOUT MAP screen
is displayed.

Selecting the NO or CANCEL button displays the REAGENT LAYOUT MAP:LOAD
REAGENTS
screen and the program is not saved.

Reagent Layout Map Screen

The REAGENT LAYOUT MAP documents the rack positions of the reagents including the fill volume
needed for completing the staining run.

The REAGENT LAYOUT MAP displays a maximum of 32 reagent vials per rack. The abbreviations
for reagents are displayed in their appropriate alpha- numeric rack positions.
















Each reagent position is color coded to correspond to Dako reagent colors and the colors from the
PROGRAMMING GRID.

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