Dako Autostainer - Manual (Page 47)

Dako Autostainer | User Guide 47
Substrate-Batch
This step splits the staining run into two distinct operations. The first includes all steps prior to the
application of the substrate and the second batches all steps starting with the substrate
application. This feature allows you to use unstable substrates that need to be prepared
immediately prior to application. The Autostainer stops after the steps in the first operation (non-
Substrate protocol steps) are completed. An audible signal indicates when the substrate should be
loaded and the screen displays where it should be placed (typically in position D8 of reagent rack
#1).
NOTE: The Substrate-Batch step can only be used once in a Protocol Template.
Switch (*Swtch)
This step programs the switching of waste disposal from one container to another for the
separation of hazardous from non-hazardous waste. The Switch step is displayed in the
Programming Grid as a “skull and cross bones” icon in each programmed row. The Switch step
can be used multiple times in any Protocol Template. When programming successive switch steps
the “skull and cross bones” icon will be alternated with a “flower” icon to indicate switching from
hazardous to non-hazardous waste. The switch icons are printed out on the Main Grid printout as
“skull and cross bones” or flower symbols.
NOTE: The switch step must be preceded by a rinse step in the protocol template.
NOTE: The first switch step in any protocol template will be a hazardous waste switch.
Creating and Editing a Protocol Template
NOTE: Low level security does not allow access to creating or editing Protocol Templates.
1. Select the PROTOCOL TEMPLATE button on the PROGRAMMING GRID. The
PROTOCOL TEMPLATE DESIGN screen appears with the default protocol template
displayed in the Protocol Outline column.
2. Select the NEW TEMPLATE button. The default Protocol Template disappears and the
default first step (Rinse Buffer) is displayed.
NOTE: If No Pre-Rinse is selected at the OPTIONS screen, then the first Rinse Buffer is
not displayed. (See Initializing the Dako Autostainer, section 7)
3. Build the new protocol template from the Protocol Elements list. To select a reagent move
the mouse pointer over the protocol element and press the left mouse button. The item is
copied to the Protocol Outline column and sequentially numbered.
NOTE: If Add Rinse to Protocol Steps is selected at the OPTIONS screen (see
Initializing the Autostainer, section 7), then rinses will be automatically added after each
step, except following a Substrate step.
NOTE: Buffer/Water rinses and hazardous/non-hazardous switches are represented in
the protocol outline with the appropriate icon for easy differentiation between the different
selections.
4. To delete a step, select it in the Protocol Outline box. The name is highlighted. Select the
DELETE STEP button. The step is removed.