Immunohistochemical report, Shutting down the autostainer – Dako Autostainer User Manual

Page 94

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94

Dako Autostainer

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User Guide

NOTE: If the Autostainer is stopped when there is reagent in the probe, it will ask you if
you would like to clean the probe. Select yes if it is safe to allow the arm to move. If
there is as object in the pathway of the arm or if the arm has been moved out of
calibration during the run, select No.

NOTE: If the ESC key is pressed during Autostainer operation, this will emergency stop
the run. Select the YES button to stop the run. Select the NO button if you do not want
to abort the run.

Immunohistochemical Report

An Immunohistochemical Report is generated for every staining run and can be printed either by
selecting the PRINT IHC button on the RUN LOG screen, or by selecting the PRINT button on the
PROGRAMMING GRID and choosing IHC option.

The IHC Report format is selected during Autostainer initialization. This format can be changed when
necessary by selecting the OPTIONS button from the INITIALIZE screen. (see Initializing the Dako
Autostainer, Section 6)

The IHC Report can be configured to print in one of the following formats:

• The One case/page option prints one case on each page of the report.

• The One slide ID/page option prints each Slide ID on a separate page.

• The No page break option prints all slides for a run in a continuous print out.

The IHC Report groups all slides by case number and prints the following information for every slide:

• slide

#

• primary antibody information (Full name, Species reactivity, Catalog #, Lot # (if entered),

Expiration date, and Incubation time)

• full name and incubation time for all reagents used

The report includes a Staining Signal/Noise and a Comments section for grading the individual slides
at the microscope. A Case Interpretation section is also provided at the end of a case’s slides to
record the pathologist’s comments. Each IHC Report is uniquely identified by the header information
which includes the date of the report, the institution information, the name of the technician and the
slide identification, case #, block ID, tissue and doctor for every case included in the current run.

Shutting Down the Autostainer

1. Select

the

SIGN OFF button on the MAIN MENU screen.

2. A dialog box appears asking: “Quit using Autostainer?” Select the YES button.

3. Move the mouse pointer to the lower left corner until the Windows “Start” icon appears.

Press the left mouse button once. A menu of items is displayed.

4. Select

Shut Down”. The SHUT DOWN WINDOWS window appears.

5. Select the “Shut down the computer” option.

6. Select

the

YES button. The computer begins its shut down procedure. Wait until the

computer displays “It’s now safe to turn off your computer”.

7. Turn off the power on the surge protector.


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