Dako Autostainer - Manual (Page 72)

72 Dako Autostainer | User Guide
5. If the primary antibody is a step that is preformed off the Autostainer (i.e. overnight
incubation or requires heating) then check the Manual Step check box by pressing the
SPACE BAR. When this reagent is selected at the PROGRAMMING GRID, “(Manual
Step)” is displayed in the reagent tile, signifying that this step is preformed before the
slides are loaded on the Autostainer. Press TAB or ENTER. The cursor moves to the
Compatibility box.
6. Enter the correct compatibility code and press TAB or ENTER. Assigning compatibility
information to reagents is optional. (See Compatibility Check at the end of this section).
The cursor moves to the Lot box.
7. Enter the reagent lot number and press TAB or ENTER. The lot # cannot exceed 8 alpha-
numeric characters. Assigning lot number information for reagents is optional. The cursor
moves to the Exp. Date box.
8. Enter the reagent expiration date and press TAB or ENTER. The reagent expiration date
entry is optional. To enter, use XXYY where XX is a two-digit numeric character
symbolizing a month and YY signifies a year. A maximum of 5 characters can be entered.
The entry of expired reagents is not accepted by the software. The cursor moves to the
Time box.
NOTE: All expiration dates entered are limited to a maximum of 10 years from the date of
entry.
9. Enter the reagent incubation time and press TAB or ENTER. The cursor moves the
Pretreatment section.
10. Select the Pretreatment from the box by using the UP and DOWN arrows. When the
correct pretreatment is listed in the text box, press TAB or ENTER. The pretreatment
short name, manual step, and incubation time will appear in the proper text boxes. The
cursor moves to the Short Name box.
NOTE: To add a new pretreatment, type the name of the pretreatment name, and press
TAB or ENTER. The cursor moves to the Short Name box.
11. Make any necessary changes to the pretreatment Short Name, and press TAB or
ENTER. The cursor moves to the Manual Step Check box.
12. Make any necessary changes to the Manual Step check box by pressing the SPACE
BAR. If the pretreatment is a step that is preformed off the Autostainer (i.e. Target
Retrieval) then select the Manual Step check box. When this reagent is assigned at the
PROGRAMMING GRID, “(Manual Step)” is displayed in the reagent tile, signifying that
this step is preformed before the slides are loaded on the Autostainer. Press TAB or
ENTER. The cursor moves to the Time box.
NOTE: Manual Step setting can also be changes from the Programming Grid, see
Programming Slides, section 10.
NOTE: A minimum of 2 minutes incubation time is required for proper operating
conditions.