Dako Autostainer User Manual
Page 114
114
Dako Autostainer
|
User Guide
Symptom
Possible Cause
Solutions
No Staining
Insufficient reagent in reagent
vial.
The Autostainer is equipped
with a liquid level sensor. If the
sensor detects insufficient
reagent to complete the current
step, a message appears on the
screen prompting you to add
missing reagent to the correct
reagent vial. You have one
minute to add reagent after
which some slides may be
skipped. Check Run Log for
“Insufficient Reagent **Skipped
Slides**” message.
Reagents vials were not loaded
in the correct locations in the
reagent racks.
Check the Reagent Map to
verify proper locations. Check
Run Log for “Unable to Locate
Reagent **Skipped Slides**”
message.
Slides were not loaded in the
correct locations.
Check the Load Slide printout to
verify proper locations.
Slides were loaded with tissue
side down.
Orient slides properly.
Programming error.
Check the programming grid to
ensure that the staining run was
programmed correctly.
Slides drying out.
Check that adequate volume is
programmed to dispense on
each slide that is suitable for
desired incubation times.
Ensure that no extreme
environmental conditions are
contributing to the drying of the
slides (i.e. direct sunlight,
heating/air conditioning vents,
hoods, etc.)