Dako Autostainer - Manual (Page 71)

Dako Autostainer | User Guide 71
Adding Primary Antibodies
A primary antibody can be added to the reagent list with a specific pretreatment reagent. This will
facilitate automatic selection of the pretreatment whenever a primary antibody is selected at the
PROGRAMMING GRID.
1. From the PROGRAMMING GRID select Edit lists from the menu bar. The reagent lists
categories are displayed.
2. Select the Primary Antibody category. The Primary Antibody EDIT REAGENT LIST
screen is displayed with the cursor in the text box in the Antibody section.
NOTE: All symbol characters such as (*), (:), (/) and (-), etc. are incompatible with the
Autostainer software. Do not use any symbol characters. The use of symbol characters
may interrupt the Autostainer during operation.
3. Type the full name of the antibody you want to add to the current list and press TAB or
ENTER. The software allows the entry of 32 alpha-numeric characters for primary
antibodies. No symbols can be used in the reagent long name. Once the box is filled an
audible signal alerts that no additional characters can be entered. The cursor moves to
the Short Name box.
NOTE: The Dako programmed list of primary antibodies includes matched negative
controls for all primary antibodies listed. The name of a negative control is that of its
corresponding primary antibody with the prefix “_NC”. For example, the negative control
for p53 is _Ncp53.
NOTE: To add a matching negative control be sure to use the exact same name as the
positive control and add the prefix “_NC” to the beginning of the reagent name. Matching
negative controls are necessary for the use of the Neg.Ctl tool. (See Programming Slide,
section 10)
4. Enter an abbreviated name for the reagent in the short name box and press TAB or
ENTER. The abbreviated name cannot exceed 10 alpha-numeric characters and should
be unique to distinguish it from other reagents. No symbols can be used in the reagent
short name. The cursor moves to the Manual Step check box.