Dako Autostainer - Manual (Page 66)

66 Dako Autostainer | User Guide
2. Selecting multiple dispense locations:
To assign multiple dispense locations on a slide, press the CTRL key while clicking on the
desired dispense locations with the mouse pointer. Multiple yellow highlights appear on
the slide identifying the selected dispense locations.
NOTE: Each of the selected dispense locations will receive the volume of primary
antibody programmed. The maximum total dispense volume per slide is 800 µL.
Assigning Reagent Dispense Volumes
The default reagent dispense volume will be applied for the primary antibody unless the dispense
volume is changed.
1. Move the mouse pointer over the dispense volume in the SLIDE LAYOUT
MAP:PROGRAM SLIDES screen and press the left mouse button. A reagent dialog box
displays the dispense volume choices.
2. Select the dispense volume by clicking in the diamond of the desired dispense volume. A
smaller black diamond appears.
3. Select the OK button or press ENTER. The SLIDE LAYOUT MAP screen is displayed
and the new reagent dispense volume and all slide information appear in red letters on
the slide.
Select CANCEL and the reagent volume is not changed.
NOTE: If the primary antibody volume is changed in the SLIDE LAYOUT
MAP:PROGRAM SLIDES screen, all other reagent dispense volumes for the specific
slide are adjusted in proportion. For example, the default volume is 200 µL of reagent
for each step. If the primary antibody dispense volume is changed to 100 µL, the
dispense volume of all other reagents will also be reduced to 100 µL.