Dako Autostainer - Manual (Page 113)

Dako Autostainer | User Guide 113
Symptom Possible Cause Solutions
Reagent probe is not
dispensing reagent. (Cont.)
Clogged reagent probe.
Contact your local Dako
representative.
(See Dako Companies and
Distributors, Section 23)
Bubbles on the surface of the
reagent in the reagent vial.
Remove bubbles.
The yellow 3- way “stopcock”
valve on the syringe has been
moved.
Verify that the “OFF” position of
the valve is in the down
direction. The valve should be in
a “T” configuration.
Z-head assembly is hitting slide
racks during priming or during
run.
Arm is out of alignment.
Emergency stop the run and
restart the run. Remove steps
already performed. Re-check
Reagent Layout Map as this
may cause vials to be
reassigned to new locations.
Blow head/wash head is bent.
Contact your local Dako
representative.
(See Dako Companies and
Distributors, Section 23)
The probe and wash/blow head
are not going to the correct
locations
The arm has been knocked out
of calibration.
Abort run and save run log.
Reprogram the run deleting out
the steps from the Protocol
Design screen that have already
been completed. Restart run.
Bent probe tip.
Verify that all reagent vial caps
have been removed and slide
racks are firmly locked in place
in the down position. Contact
your local Dako representative.
(See Dako Companies and
Distributors, Section 23)