Dako Autostainer User Manual

Page 60

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60

Dako Autostainer

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User Guide

Replacing a reagent with skip step (no reagent added to slide):

1. Move the mouse pointer over the reagent step to be changed and press the left mouse

button. The list of available reagents is displayed.







2. Select (none). (none) is displayed in the tile.

Editing a reagent in a staining step for one run only:

1. To edit a reagent in a staining step or to use a reagent not listed in the reagent list, click

on the step and select the (edit slide) item from the reagent list. The EDIT INDIVIDUAL
SLIDE REAGENT
box displays the information associated with the reagent.

NOTE: Lot and date cannot be edited here and are grayed out. Only the reagent long
name, short name, compatibility, and incubation time can be edited.









2. Add a new reagent by typing in the appropriate information. Select the OK button. The

new reagent is added for the current staining run only and will not be added to the
available list of reagents for this step. A dialog box appears asking if the new reagent is to
be assigned to all following unprogrammed fields of the reagent column.

NOTE: If there are no remaining unprogrammed fields of the reagent column, this dialog
box will not appear.

NOTE: If the newly added reagent is a primary antibody, the dialog box “Assign to all
following unprogrammed slides” does not appear.

3. Select

the

YES button and all subsequent unprogrammed fields of the reagent column

are filled with the edited reagent.

Select the NO button and the PROGRAMMING GRID appears with only one slide edited.

NOTE: Do not use symbols when entering reagent information. Doing so may cause
errors during the run.

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