Dako Autostainer - Manual (Page 112)

112 Dako Autostainer | User Guide
Symptom Possible Cause Solutions
Buffer or DI Water does not
flow out during a run or a prime.
(Cont.)
Inlet filters are clogged and are
restricting fluid flow.
Check the inlet filter at the end
of the tubing in the carboy -
clean if necessary. Dako
recommends using a tube and
cap assembly (S3472) to
prevent foreign objects from
entering the carboys and
possibly clogging the filter.
Tubing not completely inserted
into carboy.
Insure that buffer and DI water
tubing is fully inserted into the
proper carboys.
Faulty buffer / DI water pump.
Contact your local Dako
representative.
(See Dako Companies and
Distributors, Section 23)
All waste collects into one
waste container.
“Switch” is not programmed in
the Protocol Template.
Program a “Switch” in the
Protocol Template.
Waste tubing not placed in the
proper waste carboy.
Insure that all waste tubes lead
to proper carboy.
Faulty waste pump.
Contact your local Dako
representative.
(See Dako Companies and
Distributors, Section 23)
Waste backs up in the sink.
Clogged sink filter.
Clean the filter when the
instrument is not in use.
Using a small brush and
some water, remove the
material causing the
blockage.
Reagent probe is not
dispensing reagent.
Insufficient reagent in reagent
vial.
The Autostainer is equipped
with a liquid level sensor. If the
sensor detects insufficient
reagent to complete the current
step, a message appears on the
screen prompting you to add
missing reagent to the correct
reagent vial. You have one
minute to add reagent after
which some slides may be
skipped.