Dako Autostainer - Manual (Page 107)

Dako Autostainer | User Guide 107
Dako Phosphate Buffered Saline
Dako Phosphate Buffered Saline Solution can be used for Autostainer reagent preparation and
general laboratory use.
Intended Use
Dako recommends phosphate buffered saline (PBS) in the preparation of many immunohisto-
chemical and in situ hybridization procedures.
Materials Required
1. Clean carboy, flask or beaker graduated to 1 Liter
2. Deionized water
3. Magnetic stir plate with stir bar
4. Prepackaged Dako reagents
Code No. Contents
S3024 Six packets of PBS containing sodium phosphate dibasic, sodium phosphate
monobasic, and sodium chloride. Each packet makes 1 liter of 20mM sodium
phosphate, 150mM NaCl, pH 7.0.
Reagent Preparation
One Liter of Phosphate Buffered Saline Solution (S3024)
1. Add l L (1000 mL) deionized water a 1L carboy, flask or beaker.
2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the
PBS granules.
3. Add 0.5 mL Dako Tween 20 (Code No. 1966) to the phosphate buffered saline solution
and mix using a magnetic stir plate.
4. The final solution is 20mM sodium phosphate, 150mM NaCl, phosphate buffered saline,
pH 7.0 with 0.05% Tween 20.
Shelf Life and Storage
PBS solutions should be stored at 2-8° C to inhibit bacterial growth. PBS solutions which becomes
turbid should be discarded.
If phosphate buffered saline is stored at 4° C, the expected shelf life is 7 days.
If phosphate buffered saline is stored at room temperature, the expected shelf life is 4 days.
NOTE: Dako PBS does not contain any preservatives. It is recommended that the wash buffer
carboy is washed according to good laboratory practices each time before it is filled with fresh
buffer. Also, it is recommended that the end piece of the in-line buffer tubing is wiped clean
periodically to avoid clogging.