Partial reagent application – Dako Autostainer User Manual
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Dako Autostainer
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User Guide
Partial Reagent Application
Reagent vials are loaded on the Autostainer and a run has been started.
1. The reagent probe senses insufficient or a missing reagent. The MORE REAGENT
window is displayed on the screen. The screen states the reagent vial position, amount
needed and reagent that is missing.
2. A 60 second count begins to count down and an alarm sounds.
3. If the user does not notice the MORE REAGENT alarm, after 60 seconds the RUN LOG
screen appears and the pause in the run is recorded.
4. The Autostainer pumps and dispenses all the reagent available. A Low Reagent button
appears at the right of the screen. Selecting this button exposes the ADD REAGENT
window (reagent layout map) with the low reagent location and volume displayed.
5. Once sufficient reagent has been added to the vial, select the BACK button or click on
the reagent circle in the ADD REAGENT window. The run will continue.
6. If the initial MORE REAGENT error screen is not noticed, a second MORE REAGENT
screen is displayed. This screen informs the user how much additional reagent is needed
for the run. This message is displayed for 30 seconds.
7. If the user does not notice the MORE REAGENT alarm, the RUN LOG screen appears
and the pause in the run is recorded. Also listed on the RUN LOG screen is:
The amount of reagent not present.
The number of slides the Autostainer was able to process with the amount of
reagent present.
Which slides have a partial amount of reagent and how much was applied to
each slide.
Any slides that were skipped.
8. When the run is completed, the user is prompted with a list of slides that have a partial
application of reagent and which slides were skipped. This list can be printed by selecting
PRINT.