Cleaning log, Reservoir cleaning, Wash buffer and water container cleaning – Dako Autostainer Plus User Manual

Page 100

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Dako Autostainer Plus

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User Guide

NOTE: Do not use bleach in the Dako Autostainer Plus. Bleach may react with other
chemicals and create toxic fumes.

NOTE: If running protocols that require more frequent cleaning (i.e., using hematoxylin
counterstain or high salt buffers), Dako recommends performing a cleaning run a the end
of each day the instrument is used.

Cleaning Log

Each time the Autostainer Plus is cleaned, this is logged in a Cleaning Log so that a record of each
cleaning is maintained. Each time a cleaning is reset without performing an actual cleaning run,
this is recorded in the Cleaning Log with the notation “(Reset)”.

1. At the AUTOSTAINER PLUS MAINTENANCE screen, select the CLEANING LOG button.

The CLEANING LOG screen is displayed.

This screen displays every cleaning performed on the Autostainer Plus according to date
and time it was cleaned, the user who performed the cleaning, slides since the last clean,
and total number of runs on the instrument at time of cleaning.

2. To print the entire report, select the PRINT ALL button. To print a section of the report,

use the scroll bar to scroll to the section to be printed and select the PRINT SCREEN
button.

NOTE: A scroll bar will only appear when the Cleaning Log becomes too long to be
displayed on one screen.

Reservoir Cleaning

1. Pour 100 mL of deionized water into the instrument sink before starting an automated

cleaning cycle.

2. Wipe sink dry with a non-abrasive cloth or paper towel after cleaning cycle is completed.

NOTE: Ensure no particles remain in the sink after cleaning.

NOTE: Do not use bleach in the Dako Autostainer Plus. Bleach may react with other
chemicals and create toxic fumes.

Warning: Residues of hazardous reagents may be present in the sink basin and tubing
when rinsing or cleaning the Dako Autostainer Plus. Wear appropriate personal protective
equipment to prevent exposure. Hazardous waste must be disposed of according to local,
state or federal regulations. Refer to the reagent-specific Material Safety Data Sheet
(MSDS) for additional information.

Wash Buffer and Water Container Cleaning

1. Dako Autostainer Plus Wash Buffer and deionized water do not contain any

preservatives. The wash buffer and deionized water container should be washed
according to good laboratory practices each time before they are filled with fresh buffer or
deionized water.

2. Also, the end piece of the in-line buffer tubing should be cleaned to avoid clogging.

NOTE: Do not use bleach in the Dako Autostainer Plus. Bleach may react with other
chemicals and create toxic fumes.

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