Dako Autostainer Plus - Manual (Page 53)

Dako Autostainer Plus | User Guide 53
Section 10 | Programming Slides
The Dako Autostainer Plus can process a maximum of 48 microscope slides in a single staining run.
Each slide can be stained using an independently designed protocol. After the slide information is
entered and protocol template is selected, specific reagents are assigned to each slide. This is
accomplished from the PROGRAMMING GRID. The following section describes how to program the
specific reagents for a staining run.
Defining Slide Specific Reagents
Defining Detection Reagents (Secondary, Tertiary, Substrate, etc.)
1. Enter slide information and select a protocol template as described previously (see
sections 8 and 9). The PROGRAMMING GRID is displayed listing the slides identified
during Slide Count or Slide Information entry. The first programmable field is highlighted
(blinking) and a list of available reagents for the step is displayed.
2. Select the desired reagent from the list using the UP and DOWN arrow keys on the
keyboard, and press enter. Use the mouse pointer to insert the desired reagent by
clicking over the name. A dialog box prompts “Assign to the following unprogrammed
slides?”.
Select YES to assign the selected reagent to the remaining slides in the grid and the next
programmable step is highlighted.
Select NO and the next programmable step is highlighted without assigning the reagent
to the remaining slides.
3. Select the NEXT button to complete the slide programming after all reagents are
programmed. The PROGRAMMING GRID will be checked for missing rinses. If there is a
rinse missing between two reagents, a message will ask if you want to continue.
Select YES and a dialog box prompts “save program on disk?” if the program is new.
Otherwise the PRINT SLIDE LABELS window is displayed.
Select NO to return to the PROGRAMMING GRID and the missing rinse will appear on
the screen.