Dako Autostainer Plus - Manual (Page 56)

56 Dako Autostainer Plus | User Guide
4. A compatibility code must be entered. Enter the correct compatibility code for the
antibody (see Compatibility Check, Reagent Management section).
5. Assign the Universal Negative Control by selecting the Neg.Ctl Type. Select UNC or UNC
plus. A UNC is assigned to any Dako N-series ready-to-use antibodies for detection
systems such as LSAB2 or EnVision. A UNC plus is assigned to any Dako NP-series,
high sensitivity antibodies for detection systems such as LSAB+ or EnVision+.
NOTE: Four Universal Negative Controls exist in the reagent list:
• UNC Universal Neg. Ctrl. Ms N1698
• UNC Universal Neg. Ctrl. Rb N1699
• UNC plus Universal Neg. Ctrl. Plus Ms NP001
• UNC plus Universal Neg. Ctrl. Plus Rb NP015
The controls are preloaded on the instrument or added during an upgrade.
Using the Negative Control Tool
To insert a Universal Negative control, click on the NEG.CTL button in the PROGRAMMING GRID
screen (see Assigning Positive and Negative Control Reagents, Programming Slides section).
NOTE: The NEG.CTL tool selects one of the four Universal Negative Controls based on the
compatibility and negative control type defined for the primary antibody. If the Primary Antibody
does not have either a compatibility or negative control type defined, an error will appear stating
that there is not matching negative control reagent.
NOTE: After the NEG.CTL or POS.CTL feature is used, the button will disappear until the next
primary is programmed.
NOTE: If you do not wish to use this feature noted above, then continue programming as usual.
This feature is not active when using Auto Program.
Defining Specific Rinses
Rinses are automatically added following every programmed reagent (except for Protein Block; a
blow step is added following a Protein Block) when assigned as described above. The type of rinse
is selected in the Protocol Template (see Designing a Protocol, section 9).
The type of rinse can also be changed from the PROGRAMMING GRID.
1. In a rinse column, position the mouse pointer over an activated tile and click the left
mouse button. Each time the button is pressed, this will change the rinse to a different