Dako Autostainer Plus - Manual (Page 72)

72 Dako Autostainer Plus | User Guide
NOTE: Manual Step setting can also be changes from the Programming Grid, see
Programming Slides, section 10.
NOTE: A minimum of 2 minutes incubation time is required for proper operating
conditions.
13. Enter the reagent incubation time and press TAB or ENTER.
NOTE: If Manual Step was selected, the incubation time will be “0”. Do not change this
time.
The cursor moves to the first text box to allow addition of the next primary antibody.
Follow steps 3-13 until all antibodies have been added.
NOTE: If a new pretreatment was used for this antibody, message appears asking if this
pretreatment should be added to the Pretreatment list. Select Yes to add reagent to the
pretreatment list.
14. Select the OK button to add the new antibodies to the reagent list. The system prompts
“Save changes on disk?”. Select the YES button and the new antibodies are saved to the
Primary Antibody list. Select the NO button and the new antibodies are not saved. The
PROGRAMMING GRID is displayed.
Select the CANCEL button to escape without saving. The PROGRAMMING GRID is
displayed.
Editing Reagent Lists
1. From the PROGRAMMING GRID select Edit lists on the menu bar. The reagent list
commands are displayed.
2. Select the reagent category. The corresponding EDIT REAGENT LIST screen is
displayed with the cursor in the Reagent Name box.
3. Display the specific reagent by using the scroll bar or press the UP or DOWN key on the
keyboard until the target reagent appears highlighted. Press ENTER or click the left
mouse button and reagent information is displayed. Appropriate negative controls are
listed at the end of each primary antibody list. Use the list scroll bar or type “_” and
software moves the highlight to the negative controls.
4. Edit fields by highlighting the information and typing over it. When changing the reagent
name, the software saves the change as a new reagent.
NOTE: No symbols should be used in the addition or editing of reagents.
5. Select the OK button or press ENTER until a dialog box prompts “Save changes on disk?”
6. Select YES to save the changes.
Select the NO button and the reagent is not edited. The PROGRAMMING GRID is
displayed.
NOTE: Changes saved in a reagent category using Edit Reagents Lists will be reflected
in the appropriate reagent list displayed during subsequent programming sessions.