Dako Autostainer Plus - Manual (Page 83)

Dako Autostainer Plus | User Guide 83
Section 14 | Starting a Run
After the specimen-containing slides and reagent vials have been loaded and scanned, it is advisable
to go through the following checklist:
Slides
• should be flooded with buffer containing 0.05% Tween 20 using a squirt bottle to prevent
slides from drying while loading additional slides.
• should be seated securely in slide rack.
Racks
• should be properly seated in the Autostainer Plus.
Reagents
• should be at room temperature.
• should be placed at proper locations in the reagent racks.
• should contain no air bubbles.
• should contain at least the reagent volume in each vial as specified in the Reagent Layout
Map.
Buffer
• reservoir should contain at least the volume of buffer indicated in the Reagent Layout Map
plus any additional volume listed for the completion of a delayed run.
Water
• reservoir should contain at least the volume of water indicated in the Reagent Layout Map.
Waste
• containers should have enough room to accommodate the volume of buffer and water used
during the staining run.
After the above checks have been made the Start Run sequence can be initiated.
The Dako Autostainer Plus enables the user to add Priority Slides during a run. See instructions later
in this section.
Starting a Run without Delay
Starting a staining run immediately.
1. When the bar code feature is disabled, select the NEXT button on the SLIDE LAYOUT
MAP:LOAD SLIDES screen during slide loading. The SET START TIME window is
displayed. When the bar code feature is enabled, the SET START TIME window is
displayed immediately after the slides have been scanned in the LOAD AND SCAN
SLIDES screen.