Dako Autostainer Plus - Manual (Page 84)

84 Dako Autostainer Plus | User Guide
The Start Time is listed as +0:01 and the current time.
The Finish Time is listed as the +HH:MM required to complete the run. The calculated
finish time is also displayed.
2. Select the PRIME PUMP (Water) button. A dialog box appears asking if the pathway is
clear for the arm to move.
Select the YES button. The Autostainer Plus calibrates the reagent dispensing
mechanism and primes the water-dispensing pump.
Select the NO button and the SET START TIME window is displayed.
NOTE: The “01” hardware version of the Dako Autostainer Plus does not have an
additional reservoir for deionized water. If your Autostainer Plus does not have this option
the software does not prompt to prime the water pump.
3. Select the PRIME PUMP (Buffer) button. The buffer dispensing pump is primed.
NOTE: If this step is omitted prior to start of the run, the pump will be automatically
primed upon starting the run.
4. Select either the Check Volumes or Do Not Check Volumes options located near the
bottom of the screen.
NOTE: If the Check Volumes option is selected, the Autostainer Plus will check each
required reagent vial to verify that the minimum amount of reagent is available at the start
of the run. Extra buffer will be required for this function to clean the probe after checking
each reagent. When this option is selected, the amount of required buffer will increase
and you will be prompted to add extra buffer at the start of the run.
If the reagent volume is insufficient to complete the run, the Autostainer Plus will stop and
prompt the user with a message indicating that adequate reagent was not found. The
Autostainer Plus will sound an alarm and will not proceed until the user returns and
selects OK. The instrument will then resume checking volumes.
If the Do Not Check Volumes option is selected, the Autostainer Plus will not check
reagent volumes prior to the start of the run.
5. Select the START RUN button. The RUN LOG screen is displayed. The Run Log is
visible throughout the run and displays the steps performed by the Dako Autostainer Plus.
The Elapsed, Remaining, Total, Start, Current, and Finish times are listed on the RUN
LOG screen. If you need to add one or more Priority Slides during the run, select that
button (see instructions later in this Section).