Dako Autostainer Plus - Manual (Page 108)

108 Dako Autostainer Plus | User Guide
Shelf Life and Storage
TBS solutions should be stored at 2-8?C to inhibit bacterial growth. TBS solutions that become
turbid should be discarded.
If Tris-buffered saline is stored at 4?C, the expected shelf life is 7 days.
If Tris-buffered saline is stored at room temperature, the expected shelf life is 4 days.
NOTE: The above formulation for Tris-buffered saline does not contain any preservatives. It is
recommended that the wash buffer carboy is washed according to good laboratory practices each
time before it is filled with fresh buffer. Also, it is recommended that the end piece of the in-line
buffer tubing is wiped clean periodically to avoid clogging.
Dako Phosphate Buffered Saline
Dako Phosphate Buffered Saline Solution can be used for Autostainer Plus reagent preparation and
general laboratory use.
Intended Use
Dako recommends phosphate buffered saline (PBS) in the preparation of many
immunohistochemical and in situ hybridization procedures.
Materials Required
1. Clean carboy, flask or beaker graduated to 1 Liter
2. Deionized water
3. Magnetic stir plate with stir bar
4. Prepackaged Dako reagents
Code No. Contents
S3024ix Packets of PBS containing sodium phosphate dibasic, sodium phosphate
monobasic, and sodiumchloride. Each packet makes 1 liter of 20mM sodium
phosphate, 150mM NaCl, pH 7.0
Reagent Preparation
One Liter of Phosphate Buffered Saline Solution (S3024)
1. Add l L (1000 mL) deionized water a 1 L carboy, flask or beaker.
2. Empty entire contents of one packet. Use a magnetic stir plate to mix and dissolve the
PBS granules.
3. Add 0.5 mL Dako Tween 20 (Code No. 1966) to the phosphate buffered saline solution
and mix using a magnetic stir plate.
4. The final solution is 20 mM sodium phosphate, 150 mM NaCl, phosphate buffered saline,
pH 7.0 with 0.05% Tween 20.