Dako Autostainer Plus - Manual (Page 110)

110 Dako Autostainer Plus | User Guide
4. Once pH 7.0 is reached, add 0.5 mL of Tween 20 and mix using a magnetic stir plate.
5. Adjust the final volume of buffer to 1 liter with deionized water.
6. The final solution is 20mM Phosphate, 150mM NaCl, buffered saline, pH 7.0 with 0.05%
Tween 20.
Shelf Life and Storage
PBS solutions should be stored at 2-8° C to inhibit bacterial growth. PBS solutions that become
turbid should be discarded.
If phosphate buffered saline is stored at 4° C, the expected shelf life is 7 days.
If phosphate buffered saline is stored at room temperature, the expected shelf life is 4 days.
NOTE: The above formulation for phosphate buffered saline does not contain any preservatives. It
is recommended that the wash buffer carboy is washed according to good laboratory practices
each time before it is filled with fresh buffer. Also, it is recommended that the end piece of the in-
line buffer tubing is wiped clean periodically to avoid clogging.