Section 1 | introduction, Dako autostainer plus features, Universal system – Dako Autostainer Plus User Manual
Page 9: Quick start-up, Flexible programming, Economic use, Dako autostainer plus features universal system
Dako Autostainer Plus
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User Guide
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Section 1 |
Introduction
The Dako Autostainer Plus System is an automated slide processing system compatible with currently
available reagents for the staining of paraffin-embedded and frozen tissue sections, cytospins, cell
smears and fine needle aspirates. This system is designed to automate manual staining methods
routinely used in immunohistochemistry and cytochemistry, enabling the transfer of established
protocols from the bench to the Autostainer Plus. Alteration of standard reagents or experimental
conditions may not be required.
This document is a comprehensive User Guide for the Dako Autostainer Plus System.
Dako Autostainer Plus Features
Universal System
The Dako Autostainer Plus is a universal system enabling the direct transfer of routine staining
protocols from the laboratory bench.
Quick Start-up
Dako’s Autostainer Plus Support Team installs and tests the system to ensure proper operation.
The factory-installed software includes standard reagent protocols and commonly used Dako
reagents such as Dako LSAB2, Dako EnVision +, Dako EnVision Dual Link +, and HercepTest™
visualization systems. The complete line of ready-to-use Dako N and NP-series primary antibodies
and their recommended protocols are also listed.
Flexible Programming
The Dako Autostainer Plus enables the use of slide-specific programmed or user defined
protocols. The system provides intuitive programming routines, reagent and slide loading maps
and start/finish run time displays.
You can build and save an unlimited number of protocols containing up to 35 steps (including rinse
and blow steps between reagent incubations) and 64 different reagents. A staining run can
process 1 to 48 microscope slides. Individual slides can be programmed to receive different
reagents, reagent dispense volumes and reagent dispense locations.
The Autostainer Plus also enables the addition of Priority Slides during a run, permitting the
repeated addition of new slides in unoccupied positions while maintaining the integrity of the slides
running.
Economic Use
The Dako Autostainer Plus provides the flexibility to program reagent volumes of 100, 150, 200,
400 and 600 µL for any step in a staining protocol. Each step can be programmed with a different
reagent dispense volume. In addition, waste can be segregated into hazardous and non-
hazardous collection containers, reducing disposal costs.