Dako Autostainer Plus - Manual (Page 113)

Dako Autostainer Plus | User Guide 113
Symptom Possible Causes Solutions
Reagent probe is not
dispensing reagent. Insufficient reagent in reagent
vial.
Clogged reagent probe.
Bubbles on the surface of the
reagent in the reagent vial.
The yellow 3- way “stopcock”
valve on the syringe has been
moved. The Autostainer Plus is
equipped with a liquid level
sensor. If the sensor detects
insufficient reagent to complete
the current step, a message
appears on the screen
prompting you to add missing
reagent to the correct reagent
vial. You have one minute to
add reagent after which some
slides may be skipped.
Contact your local Dako
representative.(See Dako
Companies and Distributors,
Section 23)
Remove bubbles.
Verify that the “OFF” position of
the valve is in the down
direction. The valve should be
in a “T” configuration.
Z-head assembly is hitting
slide racks during priming or
during run. Arm is out of alignment.
Blow head/wash head is bent. Emergency stop the run and
restart the run. Remove steps
already performed. Re-check
Reagent Layout Map as this
may cause vials to be
reassigned to new locations.
Contact your local Dako
representative.(See Dako
Companies and Distributors,
Section 23).
The probe and wash/blow
head are not going to the
correct locations. The arm has been knocked out
of calibration.
Bent probe tip. Abort run and save run log.
Reprogram the run deleting out
the steps from the Protocol
Design screen that have
already been completed.
Restart run.
Solutions
Verify that all reagent vial caps
have been removed and slide
racks are firmly locked in place
in the down position. Contact
your local Dako
representative.(See Dako
Companies and Distributors,
Section 23).