Dako Autostainer Plus - Manual (Page 80)

80 Dako Autostainer Plus | User Guide
2. A 60 second count begins to count down and an alarm sounds.
3. If the user does not notice the MORE REAGENT alarm, after 60 seconds the RUN LOG
screen appears and the pause in the run is recorded.
4. The Autostainer Plus pumps and dispenses all the reagent available. A Low Reagent
button appears at the right of the screen. Selecting this button exposes the ADD
REAGENT window (reagent layout map) with the low reagent location and volume
displayed.
5. Once sufficient reagent has been added to the vial, select the BACK button or click on the
reagent circle in the ADD REAGENT window. The run will continue.
6. If the initial MORE REAGENT error screen is not noticed, a second MORE REAGENT
screen is displayed. This screen informs the user how much additional reagent is needed
for the run. This message is displayed for 30 seconds.
7. If the user does not notice the MORE REAGENT alarm, the RUN LOG screen appears
and the pause in the run is recorded. Also listed on the RUN LOG screen is:
ƒ The amount of reagent not present.
ƒ The number of slides the Autostainer Plus was able to process with the
amount of reagent present.
ƒ Which slides have a partial amount of reagent and how much was applied to
each slide.
ƒ Any slides that were skipped.
8. When the run is completed, the user is prompted with a list of slides that have a partial
application of reagent and which slides were skipped. This list can be printed by selecting
PRINT.