Dako Autostainer Plus - Manual (Page 78)

78 Dako Autostainer Plus | User Guide
The buttons and their functions are:
1. The reagent volume displayed in the REAGENT LAYOUT MAP screen includes a dead
volume of at least 200 µL.
2. Load the reagent vials according to the reagent map.
NOTE: Using the Reagent Layout map information, make sure that enough reagent is in
the vials to complete the programmed run and that the reagent rack is properly seated in
its correct location and orientation.
NOTE: Check that the reagent vials have no bubbles on top of the solution. Remove any
bubbles.
The buttons and their functions are:
REAGENT LIST
Displays a detailed listing of the reagents used in the current staining
run.
NEXT When the Bar Code feature is disabled, the SLIDE LAYOUT
MAP:LOAD SLIDES screen will be displayed. When the Bar Code
feature is enabled, the LOAD AND SCAN SLIDES screen will be
displayed
BACK TO GRID Returns to the PROGRAMMING GRID.
SLIDE MAP Displays the SLIDE LAYOUT MAP:REVIEW SLIDES screen. If you are
using the Bar Code feature, you will not need this display
FIRST/SECOND RACK Displays the alternate reagent rack when more than 32 reagents are
used in a run. If more than 32 reagents are required for a run, this
button is labeled SECOND RACK and a second rack can be accessed
from this button. The background for the second rack is blue and the
vial positions appear with a “b” suffix (for example, A2b indicates
position As in the second/back rack). When the second is not available,
this button is labeled FIRST RACK and is grayed out and inactive.
PRINT Prints the reagent layout map.
Reagent List Screen
To view a detailed listing of the reagents used in the current run, click on the REAGENT LIST button
in the REAGENT LAYOUT MAP screen.
Select the PRINT button to generate a detailed reagent list.
Select the OK button to return to the REAGENT LAYOUT MAP screen.
Missing Reagent Error
The Dako Autostainer Plus prompts the user when an insufficient volume of reagent is measured for a
run. The Autostainer Plus can be paused to allow the user to add more reagent to the specified