Dako Autostainer Plus - Manual (Page 114)

114 Dako Autostainer Plus | User Guide
Symptom Possible Causes Solutions
No Staining Insufficient reagent in reagent
vial.
Reagents vials were not loaded
in the correct locations in the
reagent racks.
Slides were not loaded in the
correct locations.
Slides were loaded with tissue
side down.
Programming error.
Slides drying out.
The Autostainer Plus is
equipped with a liquid level
sensor. If the sensor detects
insufficient reagent to complete
the current step, a message
appears on the screen
prompting you to add missing
reagent to the correct reagent
vial. You have one minute to
add reagent after which some
slides may be skipped. Check
Run Log for “Insufficient
Reagent **Skipped Slides**”
message.
Check the Reagent Map to
verify proper locations. Check
Run Log for “Unable to Locate
Reagent
**Skipped Slides**” message.
Check the Load Slide printout
to verify proper locations.
Orient slides properly.
Check the programming grid to
ensure that the staining run
was programmed correctly.
Check that adequate volume is
programmed to dispense on
each slide that is suitable for
desired incubation times. Insure
that no extreme environmental
conditions are contributing to
the drying of the slides (i.e.,
direct sunlight, heating/air
conditioning vents, hoods, etc.).
The text of the label does not
begin along the left edge of the
label and the text is getting cut
off. The Seymour printer may need
to be reset.
To reset the Seymour Label
Printer, turn off the printer.
Hold down the green label feed
button on the top of the printer.
Turn on the printer while
holding down the green button
and continue holding the button
for 3 seconds. The labels
advance forward and printer will
print. Release the feed button.
Press and release the feed
button one more time to
advance one label. Perform a
TEST PRINT. The print will be
lined up along the left slide of
the label.