Dako Autostainer Plus - Manual (Page 18)

18 Dako Autostainer Plus | User Guide
Selecting the UNPRINTED LABELS button will print all labels that were not printed
through the PRINT feature and bring the user to the REAGENT LAYOUT MAP.
Selecting the NONE button will direct the user to the REAGENT LAYOUT MAP.
The CANCEL button will return the user to the PROGRAMMING GRID.
18. Load reagents using the REAGENT LAYOUT MAP.
19. Select the NEXT button. The LOAD AND SCAN SLIDES screen appears.
20. If the Bar Code feature is disabled, load slides using the SLIDE LAYOUT MAP. If the Bar
Code feature is enabled, slides may be loaded into positions at the discretion of the user.
It is recommended that each slide be flooded gently with buffer after it is placed on the
instrument. This buffer should contain 0.05% Tween 20 (see Loading Slides, Section 13).
At no time should the slides dry out.
21. Select the SCAN SLIDES button to scan all slides on the instrument.
22. The IS THE ARM CLEAR TO MOVE? Screen is displayed. Select OK to proceed. If no
obstructions exist, select OK.
23. The SET START TIME screen is displayed (see Starting a Run, Section 14). The
Autostainer Plus calculates the run time and amount of buffer and DI water needed to
complete the run.
24. Select the PRIME PUMP (WATER) button. The DELAYED START dialog box appears
(deionized water is not available with the “01” hardware version of the Dako Autostainer
Plus). The user is prompted: “Is the pathway clear for the arm to move.”
25. Select the OK button and the pump is activated. Deionized water is flushed through the
water intake line.
26. Select the PRIME PUMP (BUFFER) button and the pump is activated, flushing buffer
through the buffer intake line. For the “01” hardware version of the Dako Autostainer Plus
the user is prompted: “Is the pathway clear for the arm to move.”
27. Set Reagent Levels to Check Volumes or Do Not Check Volumes (see Starting a Run,
Section 14).
28. Select the START RUN button and the run starts.