Dako Autostainer Plus - Manual (Page 57)

Dako Autostainer Plus | User Guide 57
selection: rinse buffer, rinse (DI) water, blow, or none (for details of each option, see
Designing a Protocol, section 9).
2. In a rinse column, position the mouse pointer over a tile with a (blue) rinse buffer icon and
click the right mouse button. This will change the buffer rinse to a blow step. To change
the blow step back to a buffer rinse, position the mouse pointer over the blow icon and left
click the mouse button.
3. In a rinse column, position the mouse pointer over a tile with a (blue) rinse buffer icon and
hold down the left mouse button and click the right mouse button. Release both buttons.
This will change the buffer rinse to a DI water rinse step. To change the water rinse back
to a buffer rinse, position the mouse pointer of the water rinse icon and left-click the
mouse button.
NOTE: Rinses that precedes a switch (see Designing a Protocol, section 9) cannot be
changed from the PROGRAMMING GRID. The rinse at the end of a run cannot be
changed into a blow step.
Auto Programming
Auto Programming is a feature to make slide programming more efficient. This function is used to
save all, or any portion, of a protocol to be used in future programming.
Creating and Saving an Auto Program
NOTE: Low level security does not allow access to creating and saving Auto Programs.
1. Enter Slide Information and select a protocol template. A minimum of one slide must be
programmed through SLIDE COUNT or the SLIDE INFORMATION screen. Press OK or
the FINISH ENTRY button. The PROGRAMMING GRID is displayed.
2. Fill in the programming grid columns with specific reagents, except primary antibody for
one of the programmed slides.
NOTE: This will create an Auto Program for the detection reagents only. To create an
Auto Program for a protocol that includes the primary antibody, fill in the programming
grid columns with the specific primary antibody and detection reagents.
3. Select Auto from the menu bar on the PROGRAMMING GRID using the mouse pointer.
4. Select Setup from the Auto menu. Auto changes to Setup on the menu bar.
5. Select one or more rows of programmed tiles by clicking on the first row. Move the mouse
pointer over the desired rows while holding down the left mouse button. Each selected