Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 106)

Chapter 4. Lanes
4-9
Fig. 4-9. Profile of a defined lane.
In the lane profile, bands are represented by peaks and background intensity is
represented by the baseline region below the peaks. The profile is generated by
calculating the average intensity of each horizontal row of pixels across the specified
width of the lane.
To close the lane profile, click on Hide Overlays on the main toolbar.
4.2 Lane-Based Background Subtraction
After defining lanes, we strongly recommend that you perform lane-based
background subtraction. This is the best method for removing background intensity
from lanes, and is required for Gaussian modeling of bands.
Lane-based background subtraction uses a “rolling disk” method of subtraction,
named for a hypothetical disk that rolls along underneath the lane profile, removing
different intensity levels along the length of the lane.
The size of the disk determines how much background will be subtracted. A large
disk will follow the profile trace less closely, touching fewer points along the trace
Intensity peaks
Background