Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 245)

Quantity One User Guide
A-4
4. Optimize the display.
5. Analysis.
6. Select the output.
A.2 Step I. Position Gel
The Gel Doc window will open in “live” mode, giving you a live video display of
your sample. In this mode, the Live/Focus button will appear selected, and frames
will be captured and displayed at about 10 frames per second, depending on the speed
of your computer.
You can use live mode to zoom, focus, and adjust the aperture on the camera, while
positioning the sample within the area.
Note: Newer versions of the Gel Doc feature a motorized zoom lens that can be
controlled directly from the acquisition window using the Iris, Zoom, and Focus
arrow buttons. Click on the Up/Down buttons while viewing your sample in the
window to adjust the lens. These buttons will not be visible if you are connected
to older versions of the Gel Doc without the motorized zoom lens.
Fig. A-3. Newer Gel Docs feature camera control buttons in the acquisition window.
You can also select the Show Alignment Grid checkbox to facilitate positioning.
Note: After positioning your sample, you should check the Imaging Area dimensions
under Options (see section A.9, Options) to make sure that they conform to the
size of the area you are focusing on. To determine the size of the area you are