Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 174)

Chapter 6. Standards and Band Matching
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Fig. 6-25. Entering a dilution series.
The quantity of each band will be automatically calculated.
6.3.e Importing a Calibration Curve
A calibration curve created for one gel can be applied to other gels.
Make sure that the new gel you want to quantitate and the gel with the existing
calibration curve are both open.
Click on the new gel to quantitate. Select Quantity Standards from the Analysis
menu and click on Create New, as previously described.
In the new Quantity Standards dialog, click on the Import Curve button, and select
the existing calibration curve from the list. When you make the selection, the values
for the curve will be displayed in the new Quantity Standards dialog. Each standard
value will be labeled Import in the Band column.
Checking the Imported Curve
If the quantity of one or more bands in the new image is known, you can verify the
accuracy of the imported calibration curve. Go to Select in the Quantity Standards
dialog, click on Band, then click on a known band. Its lane number, band number, and
intensity will be displayed in one of the standard values fields.