Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 176)

7-1
7. Volume Tools
You can use the Volume tools to quantitate bands, spots, arrays, and other image data.
What is a Volume?
A volume is the total signal intensity inside a defined boundary drawn on an image.
To measure the amount of a particular object (e.g., a band or spot), you draw a volume
rectangle, contour, free hand, or circle around the object and compare the intensity
data inside the boundary with the data of other objects or a standard using the Volume
Analysis Report and Volume Regression Curve (see section 10.6, Volume Analysis
Report).
Volume = Sum of the intensities of the pixels within the volume
boundary x pixel area
Volume units = intensity units x mm
2
Volumes are similar to band contours (see section 5.8.a, Contouring Bands), except
that they are not dependent on lanes and bands.
7.1 Creating a Volume
To create a volume, select Volume Overlay Tools from the main toolbar or the Edit
menu. These commands are also located on the Volume menu.