Bio-Rad Quantity One 1-D Analysis Software User Manual
Page 169
Quantity One User Guide
6-26
The calibration curve is generated by plotting the quantities versus the intensities of
the known bands. The intensities of the bands can be measured in several different
ways. Click on the Measure button to display a list.
Fig. 6-20. Band attributes to measure for relative quantity.
The options in the Measure dialog are:
•
Trace density—The intensity of a band as measured by the area under its
intensity profile curve. Units are intensity x mm.
•
Contour density—The intensity of a band that has been defined using the
Contour or Draw Band tools (see section 5.8, Irregularly Shaped Bands in
Lanes). It is the sum of the intensities of all the pixels inside the band boundary
multiplied by the area of each pixel. Units are intensity x mm
2
.
•
Peak density—The intensity value of a band’s peak.
•
Average density—The total intensity of the rows of pixels used to generate the
profile of a band, divided by the number of rows.
Identifying the Known Bands in the Gel
The dialog includes three buttons that can be used to select the known bands in the
gel:
•
To select bands one at a time, click on the Band button, then click on each band
of known quantity. Each band will be highlighted.
•
If all of the known bands are the same band type, click on the Match button, then
click on one of the bands. The matched group of bands will be highlighted.