Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 178)

Chapter 7. Volume Tools
7-3
Fig. 7-2. Volume contour.
The contour should completely surround the data you want to quantify.
To edit the contour, position the cursor on the border. The cursor will change to a
pencil tool. Drag across the line; a new white line will appear. When you recross the
old line, a new contour will be created.
Volume Free Hand Tool
Use the Volume Free hand Tool to manually draw a volume boundary. First magnify
the band or other object (you must be able to see the individual pixels). Then click on
the Volume Free hand button and use the cursor to draw a line around the object.
When the line crosses itself, a free hand volume is created.
Fig. 7-3. Volume free hand.
If you make a mistake while drawing, backtrack with the mouse. The line you draw
should completely surround the data you want to quantify.
To edit the volume, position the cursor on the border and drag across the line; a new
white line will appear. When you recross the old line, a new free hand volume will be
created.