Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 295)

Quantity One User Guide
D-6
Next to Filter, click on either the Red, Green, Blue, or White checkbox, or a
combination of two of the first three (Red-Green, Green-Blue, Red-Blue).
Fig. D-6. Examples of filter colors and applications.
Next to Light, select the appropriate light source.
• Reflective mode is for scanning opaque mediums such as dried gels on filter
paper, arrays, TLC plates, and photographs. The scanning dimensions in this
mode are 21 cm
x 35 cm (uncalibrated).
• Transmissive mode is for scanning transparent mediums such as films, gels, and
slides. The scanning dimensions in this mode are 20 cm
x 25 cm (uncalibrated).
D.3 Step II. Select Scan Area
Preview Scan
Before selecting the particular area to scan, you can preview the entire scanning area
to determine the exact position of your sample.
Click on Preview Scan. A quick, low-resolution scan of the entire scanning area will
appear in the scanning window. Your sample should be visible within a portion of this
scan.
Filter Color Wavelength Application Examples
Red 595–750 nm Coomassie G-250, BCIP/NBT, Fast
Green FCF, Methylene Blue
Green 520–570 nm Coomassie R-250, Basic Fuchsin
Blue 400–530 nm Crocein Scarlet
Gray Scale 400–750 nm Silver Stains, Copper Stains, Film